Breast Cancer Lab Report Example | Topics and Well Written Essays - 750 words

Breast Cancer - Lab Report Example Acccording to studies,it is known that glycosylation is more pronounced in the breast cancer cells (Hakomori 1985). The reason for this is because of the increase in tne oligosaccharide structures (Hudis 2007).Such alterations might be identified through lectins which are the carbohydrate-binding proteins   an they have a very high specificity for the saccharides(Brooks and harris 2006).The lectins normally function as   the data mediators in the biological systems.In this case they interact with the glycoproteins glycolipids as well as the oligosaccharides(Nilsson 2007).They also bind specifically to the carbohydrate epitopes(Staffieri et al. 2012).The immunofluorescence method as well as the direct lectins cytochemistry   with the use of the   FITC-labeled lectins enables the visualization of the glycosylation’s impact on the therapies which targets the surface of the cells that bind to theHER2 receptors (Gabius 2009.The results from the histograms above (fig 1), ind icates the reason why the glycosylation’s intensity is higher in the sixth cell line when compared to that of the fifth cell line because of the different blocking agents used. In summary, further study needs to be done to clearly understand why there was a difference in the intensity of this two cell lines. It could be due to the above three mentioned reasons. Unfortunately, this study could not ascertain this.The reasons that could have resulted to the difference in the intesity could be due; firstly,the BSA could be a better blocking agent.